Journal of Medical Innovation and Technology <p>The&nbsp;Journal of Medical Innovation and Technology (JOMIT) is an international open access periodical officially owned by the Eskisehir Osmangazi University. The Journal is published twice a year (June - December) in English. ISSN: 2667-8977</p> <p>&nbsp;</p> en-US (Journal of Medical Innovation and Technology) (R. Bugra Husemoglu) Mon, 16 Dec 2019 00:00:00 +0000 OJS 60 Simulation with three dimensional modeling before spinal surgery and its effects on the surgery <p>Three-dimensional printers which are among the popular innovations of today are used increasingly more in the medical field as in every field of life and they make the surgical operation easier. When customizable materials are combined with technology, we have successful results and fewer complications. The objective of our study was to demonstrate the advantages provided by three-dimensional modeling methods in neurosurgery practice for both the surgeon and the patient. We reached the conclusion that 3D printing techniques were the practically and anatomically correct methods for the production of patient-specific models for surgical planning, simulation and training, tissue engineering implants and the secondary devices. Thus, the progress of this technology may contribute to the progress of the neurosurgery field in several aspects.</p> İsmail Kaya, İlker Deniz Cingöz, M. Cansu Şahin, Nevin Aydın, Hasan Emre Aydın, Ali Arslantaş, Egemen Nursoy ##submission.copyrightStatement## Mon, 13 Jan 2020 13:57:40 +0000 3D Printing Assisted Upper Thoracic Vertebral (T1–6) Fractures Instrumentation <p>Three dimensional (3D) printed models specific to the patients, decrease preoperative complications by providing preoperative surgical planning and application opportunities to the surgeon. The aim of this study is to determine the clinical results of preoperative surgical planning and application on 3D printed models, in patients with upper thoracic vertebral fracture treated via pedicle screw and posterior spinal fusion. 6 patients in total with an upper thoracal fracture from our clinics were included in this study. 3D printer with custom design printing capability and lamellar printing technology was used for the production of 3D models. Preoperative surgical planning and application were performed on the models. The mean age of the patients was 47.16±13.40 (25-64). 4 (66.7%) of 6 patients were males and 2 (33.3%) were females. All presented after a history of trauma. The mean operation duration was 253.33±74.47 minutes (170-360). The mean duration of hospitalization was 13.83±9.06 days. Spinal instrumentation with the assistance from preoperative 3D models is an effective and reliable treatment for upper thoracic vertebral fractures. The use of the 3D models increases the ability of preoperative simulation in favor of the surgeon. This technique decreases the risk of screw malposition and shortens the operative duration thus enhances intraoperative reliability.</p> Ceren Kızmazoglu, Rıfat Saygın Altınağ, Fazlı Oğuzhan Durak, Ege Coşkun, Resit Bugra Husemoglu, İsmail Kaya, Hasan Emre Aydın, Turan Kandemir, Orhan Kalemci ##submission.copyrightStatement## Mon, 13 Jan 2020 14:20:39 +0000 Investigation of Surface Adhesion of MCF-7 Cells in 3D Printed PET and PLA Tissue Scaffold Models <p>Tissue scaffolds with a wide range of applications are usually rigid structures made of polymeric materials. Biocompatibility and biodegradability are important properties for scaffold materials to possess, ensuring they support for cell growth and are extremely useful in in vitro three dimensional (3D) cell cultures.</p> <p>Cancer is a disease caused by mutations or abnormal changes in genes responsible for regulating the growth of cells and keeping them healthy. Breast cancer is the most common type of invasive cancer and the second cause of cancer death among women.</p> <p>Two-dimensional (2D) cell cultures have helped to attain important knowledge about cell biology and biochemistry. However, they&nbsp; are not suitable for clinical use. 2D in vitro studies do not provide the desired success in in vivo applications. The formation of the tumor microenvironment is challenging.</p> <p>Tissue scaffolds are 3D cell culture systems that eliminate this problem with breast cancer cell culture. Cell culture models with 3D tissue scaffold are thought to be more successful in representing in vivo. The main objective of this study was to produce biocompatible and suitable porosity scaffolds from polylactic acid (PLA) and polyethylene terephthalate (PET) materials, which enables MCF-7 breast cancer cells to proliferate in three dimensions.</p> <p>Polyethylene terephthalate (PET) and polylactic acid (PLA) are biocompatible, non-toxic dye-free polymers and are used for the production of scaffolds that are rigid structures suitable for 3D cancer cell culture. A custom 3D printer and 1.75 mm PET and PLA filaments were used for the production of tissue scaffolds. Tissue scaffolds are produced with two different filling rates (20% and 40%). The design and production parameters of the scaffolds are defined and optimized by SolidWorks and Slic3r softwares to set the correct printing procedure. Biomechanical tests for mechanical characterization of all scaffolds were performed. MCF-7 breast cancer cell line was used to evaluate tissue scaffolds for 3D cell culture. The ability of the cells to adhere to the scaffold surface was determined by crystal violet fixation and staining method used to detect viable cells. 3D cell culture with PET and PLA tissue scaffolds is useful to improve cancer cell culture applications and enhance cell proliferation. 3D tissue scaffolds have shown that MCF-7 cells are more compatible with surface adhesion than 2D cultures.</p> <p>As a result, the data obtained show that porous PET and PLA tissue scaffolds are supportive for the 3D culture and proliferation of MCF-7 breast cancer cells by providing a micro-environment in vivo mimic.</p> Öykü Gönül Geyik, Belma Nalbant, Resit Bugra Husemoglu, Zeynep Yüce, Tarkan Ünek, Hasan Havıtçıoğlu ##submission.copyrightStatement## Mon, 13 Jan 2020 14:23:28 +0000 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in vitro <p>Dermal fibroblasts are mesenchymal cells that produce extracellular matrix. Fibroblasts play an important role in the skin wound healing process and skin bioengineering. The aim of this study is to evaluate the behavior of 3D printed polylactic acid (PLA) scaffolds in terms of biocompatibility and toxicity on human dermal fibroblasts (HDFs). Scaffolds were prepared with the PLA filament using a custom made fused deposition modeling (FDM) printer. We fabricated scaffolds with two different pore sizes (35% and 40%). HDFs were seeded at different densities on PLA scaffolds. The cell growth was measured by WST-1 colorimetric assay after 12 and 18 days of seeding HDFs on 3D PLA scaffolds. The morphology and the adhesion property of HDFs were visualized by scanning electron microscopy (SEM). HDFs showed a significant cell proliferation in 3D printed PLA scaffolds. The cell proliferation was highest at a density of 4 x 10<sup>4</sup> cells per well. SEM images showed that HDFs attached the surfaces of the scaffolds and filled the inter-fiber gaps. Our results showed that PLA scaffolds fabricated by 3D bioprinting is a promising candidate for HDF seeding and could have a potential application wound healing or personalized drug trials.</p> Ufkay Karabay, Resit Bugra Husemoglu, Mehtap Yüksel Eğrilmez, Hasan Havitcioglu ##submission.copyrightStatement## Mon, 13 Jan 2020 14:25:00 +0000 Biomechanical Wear Tests in a Novel Hip Joint Simulator <p>The work in this paper involves the study of the biomechanical wear tests and joint kinematics analysis. Such analyses are very useful to investigate the mobility and natural functionality as well as the motion variation due to replacement implant. The simulator can be used in implant design. The simulator is developed to provide rotation of mobility with the intention of using it for different joints loaded under body activities. Modeling the human joint as rotation is a challenge because of the complexity of linking the motion drivers of the simulator with the constrained joint motion of the joint to be simulated which inevitably involves constraining of simulator motion. In this study, a joint simulation platform has been developed. Microcontroller, relay module, DC motor and power regulators are used in building the platform. The motion of the bottom plate of a joint simulation platform is controlled by a rotational axes control system connected to one actuator independently. The actuator is used to obtain the required load and displacement.</p> <p>This work is performed to study the mobility, wear and load analysis of the artificial hip joint. The presented platform can be used for joint simulation, implant testing, joint kinematics, laxity analysis, load transmission. The proposed simulator has been tested for validation of the human hip implant insert. The worn surfaces were observed by scanning electron microscopy (SEM). It is shown that the wear rates obtained in this work are closer to clinical studies than to similar hip joints simulator studies.</p> Bugra Husemoglu; Orkun Halac, Onur Hapa; Fatih Ertem, Ahmet Karakaşlı; Hasan Havitcioglu ##submission.copyrightStatement## Mon, 13 Jan 2020 00:00:00 +0000 Magnetoencephalography as a Clinical Tool: A Brief Review of Current Studies <p>Magnetoencephalography (MEG) is becoming a very popular functional neuroimaging tool in clinical practice. It is currently used with other imaging methods to aid diagnosis and pre-surgical mapping of many conditions ranging from epilepsy to depression. This paper reviews the most current studies that have utilized MEG for investigating some of these conditions and discusses the benefits of using this method in medical practice.</p> Huseyin Ozenc Taskin ##submission.copyrightStatement## Sat, 18 Jan 2020 17:49:53 +0000 3D Printer Assisted C1-C2 Posterior Spinal Fusion <p>Odontoid fractures often occur after trauma. The vascular and neural structures that are close in the cases planned for surgery increase the complications of the operation. With the recent technological advances, anatomic simulations with 3D printers have enabled the operation to be performed in less time with less risk in order to perform safer surgery. We performed a posterior C1-C2 fusion procedure in a 62-year-old patient who developed odontoid fracture after trauma with the help of anatomical simulation we wrote with a 3D printer and we wanted to present this case.</p> Inan Uzunoglu, R. Bugra Husemoglu, Ilker Deniz CINGOZ, Ceren Kizmazoglu, Murat SAYIN, Nurullah YUCEER ##submission.copyrightStatement## Mon, 16 Dec 2019 00:00:00 +0000 PLACE OF PORTABLE COMPUTED TOMOGRAPHY IN NEUROSURGERY PRACTICE <p>Portable computed tomography is mostly used in operating rooms, intensive care units, emergency service and interventional operation units. It provides a good diagnosis method in intensive care unit patients. It determines bleeding, hydrocephaly, cerebral edema which may occur in cranial cases. As a result, it gives early patient intervention chance.&nbsp; Radiation exposure is the greatest factor decreasing usage indication. It is predicted that the use would increase by minimalizing radiation ratio with technological developments.</p> TURAN KANDEMİR ##submission.copyrightStatement## Mon, 13 Jan 2020 14:33:35 +0000